Optical Analysis

Define the following terms: electromagnetic radiation electromagnetic spectrum frequency and wavelength. Describe in general terms the relationship between electromagnetic radiation and optical analysis. Identify the parts of a colorimeter. Explain how a colorimeter is used to analyze a sample. Explain how a calibration curve is obtained. Describe the basic principles of polarimetry. Identify the parts of a simple polarimeter. Explain how to set up and use a simple polarimeter. Describe a general procedure for performing an analysis using an automatic polarimeter. Describe the basic principles of turbidimetry and nephelometry. Describe a general procedure for using a turbidimeter to analyze a sample. Describe the basic principles of refractometry. Describe a procedure for using a refractometer. Define the term refractive index and identify factors that can affect the refractive index of a sample.