Mass Spectrometry

Define the following terms: spectrum mass spectrometer mass spectrum abundance relative abundance and base ion. Describe the basic process of mass spectrometry and explain why it can be used to perform qualitative and quantitative analyses. List the major steps associated with the process of mass spectrometry. Identify the major parts of a typical mass spectrometry system and describe the basic operation of the system. State the separate purposes of gas chromatography and mass spectrometry in a GC/MS system. List a major advantage and three limitations that relate to sample selection for a GC/MS system. Describe a typical sample introduction step for a GC/MS system. Describe the electron ionization process. Describe how fragmentation occurs in a mass spectrometer. Describe how a repeller and focusing optics can direct ions into a mass analyzer. Describe how separation by mass occurs in a quadrupole mass analyzer. Describe the use of an x-ray lens and an electron multiplier for ion collection detection and amplification. Explain how the scan mode and the SIM mode enable a mass spectrometer to scan different ranges of ions in a mass spectrum. Describe the data processing and control functions performed by the data system in a GC/MS system. Define the following terms: molecular ion parent ion isotopes and tuning. Describe the features of a typical mass spectrum. Describe the features of a total ion chromatogram. Describe lab technician responsibilities associated with a GC/MS system.